Name: Seungah Lee, Ph.D.
Email: [email protected]
Url: https://sites.google.com/view/homeofslee/
Tel: +82-31-201-3242 (Lab.)
Fax: +82-31-201-2340
(Appl. Chem, KHU)
Email: [email protected]
Url: https://sites.google.com/view/homeofslee/
Tel: +82-31-201-3242 (Lab.)
Fax: +82-31-201-2340
(Appl. Chem, KHU)
Education
- April 2013 - Present
- October 2010 - February 2013
- December 2008 - June 2010
- March 2006 - February 2010
- September 2003 - August 2005
- March 2000 - August 2003
Teaching experience
- 2007. 08 - 2007. 12
- 2007. 03 - 2007. 08
- 2006. 08 - 2006. 12
- 2006. 03 - 2006. 08
- 2003. 08 - 2003. 12
Publications
37. "Decreased Membrane Fluidity of Human Bone Marrowderived Mesenchymal Stem Cells Over Time" T. H. Shin, S. Lee, K. R. Choi, D. Y. Lee, Y. Kim, M. J. Paik, C. Seo, S. Kang, M. S. Jin, S. H. Kang, G. Lee, in revision by Sci. Rep. (2016).
36. "Optical Super-Resolution Imaging of Surface Reactions" T. Chen, B. Dong, K. Chen, F. Zhao, S. Lee, P. Zhang, S. H. Kang, J. W. Ha, W. Xu, N. Fang, in revision by Chem. Rev. (2016).
35. "Ultrasensitive Detection of Alpha-fetoprotein by Total Internal Reflection Scattering-based Super-resolution Microscopy for Super-localization of Nano-immunoplasmonics" S. Ahn, P. Zhang, H. Yu, S. Lee, S. H. Kang, Anal. Chem., 88, 11070-11076 (2016).
34. "Direct Quantitative Screening of Influenza A Virus without DNA Amplification by Single-particle Dual-mode Total Internal Reflection Scattering" S. Lee, S. K. Chakkarapani, E. S. Yeung, S. H. Kang, Biosensors and Bioelectronics 87, 842–849 (2016).
33. "Augmented 3D Super-resolution of Fluorescence-free Nanoparticles Using Enhanced Dark-field Illumination Based on Wavelength-modulation and a Least- cubic Algorithm" P. Zhang, K. Kim, S. Lee, S. K. Chakkarapani, N. Fang, S. H. Kang, Scientific Reports 6, 2863; doi: 10.1038/srep32863 (2016).
32. "Quantitative Nanoimmunosensor based on Dark-field Illumination with Enhanced Sensitivity and On-off Switching using Scattering Signals" S. Lee, H. Nan, H. Yu, S. H. Kang, Biosensors and Bioelectronics 79, 709-714 (2016).
31. "Real-time Intracellular Mg2+ Signaling and Wave Propagation by Subdiffraction-Limit Super-Resolution Microscopy" S. K. Chakkarapani, S. Lee, G. Lee, S. H. Kang, Bull. Korean Chem. Soc. 36, 2589-2595 (2015).
30. "Super-resolution of Fluorescence-free Plasmonic Nanoparticles using Enhanced Dark-field Illumination based on Wavelength-modulation" P. Zhang, S. Lee, H. Yu, N. Fang, S. H. Kang, Scientific Reports 5, 11447; doi: 10.1038/srep11447 (2015).
29. "Plasmonic Metal Scattering Immunoassay by Total Internal Reflection Scattering Microscopy with Nanoscale Lateral Resolution” S. Lee, H. Yu, S. H. Kang, Chem. Commun., 51, 945-947 (2015).
28. "Ultra-sensitive Plasmonic Nanometal Scattering Immunosensor Based on Optical Control in Evanescent Field Layer" S. Lee, G. Park, Suresh Kumar Chakkarapani, Seong Ho Kang, Biosensors and Bioelectronics 63, 444-449 (2015).
27. "Fast-High-throughput Screening of H1N1 Virus by Parallel Detection with Multi-channel Microchip Electrophoresis" Peng Zhang; He Nan; Seungah Lee; Seong Ho Kang, submitted to "Microchip capillary electrophoresis" in the series Methods in Molecular Biology, Springer (2014).
26. Selective Fluorescence and Fluorescence-Free Detection of Single Biomolecules on Nanobiochips" Seungah Lee; Seong Ho Kang, J. Biomed. Nanotechnol 10, 2620–2640 (2014).
25. Fluorescent-free Detection on Nanobiochips based on Wavelength-Dependent Single Plasmonic Nanoparticles by Differential Interference Contrast Microscopy" Seungah Lee; Seong Ho Kang, Biosensors and Bioelectronics 60, 45-51 (2014).
24. "Selective Fluorescent-free Detection of Biomolecules on Nanobiochips by Wavelength Dependent-enhanced Dark Field Illumination" Seungah Lee; Hyunung Yu; Seong Ho Kang , Chem. Commun. 49, 8335-8337 (2013).
23. "Single C-Reactive Protein Molecule Detection on a Gold-Nanopatterned Chip Based on Total Internal Reflection Fluorescence" Yunmi Heo; Seungah Lee; Sang-Won Lee; Seong Ho Kang, accepted by BKCS (2013).
22. "Gold-nanopatterned Single Interleukin-6 Sandwich Immunoassay Chips with Zeptomolar Detection Capability Based on Evanescent Field-enhanced Fluorescence imaging" Seungah Lee; Seong Ho Kang, Analyst 138, 3478-3482 (2013).
21. "Single-molecule DNA Digestion in Various Alkanethiol-functionalized Gold Nanopores" Seungah Lee; Seong Ho Kang, Talanta 107, 297-303 (2013).
20. "Quenching Effect on Gold Nano-patterned Cardiac Troponin I Chip by Total Internal Reflection Fluorescence Microscopy" Seungah Lee; Seong Ho Kang, Talanta 104, 32-38 (2013).
19. "Single-molecule Sandwich Immunoassay for Quantification of Alpha-Fetoprotein Based on Evanescent Field-enhanced Fluorescence Imaging" Seungah Lee; Seong Ho Kang, J. Nanomaterials 2012, 1-7 (2012).
18. "Wide-range Quantification of Human Thyroid-stimulating Hormone Using Gold-nanopatterned Single-molecule Sandwich Immunoassay Chip" Seungah Lee; Seong Ho Kang, Talanta 99, 1030-1034 (2012).
17. "Photoswitchable Fluorescent Diarylethene in a Turn-on Mode for Live Cell Imaging" Shi-Chong Pang; Hyesook Hyun; Seungah Lee; Daeun Jang; Min Jae Lee; Seong Ho Kang; Kwang-Hyun Ahn, Chem. Commun. 48, 3745-3747 (2012).
16. "Integrated Optical Molecular Imaging System for Four-Dimensional Real-Time Detection in Living Single Cells" Seungah Lee; Seong Ho Kang, Biosens. Bioelectron. 31, 393-398 (2012).
15. "Enzyme Digestion of Entrapped Single-DNA Molecules in Nanopores" Seungah Lee; Seong Ho Kang; Edward S. Yeung, Talanta 85, 2135-2141 (2011).
14. "Native and Fluorescent Dye-Dependent Single-DNA Molecule Microchip Dynamics as Measured by Differential Interference Contrast Microscopy" Doori Oh; Seungah Lee; Seong Ho Kang, Chem. Commun. 47, 9137-9139 (2011).
13. "Digestion of Individual DNA Molecules by λ-Exonuclease at Liquid-Solid Interface" Seong Ho Kang; Seungah Lee; Edward S. Yeung, Analyst 135, 1759-1764 (2010).
12. "Detection of Single Enzyme Molecules Inside Nanopores Based on Chemiluminescence" Seong Ho Kang; Seungah Lee; Edward S. Yeung, Angew. Chem. 122, 2657-2660 (2010); Angew. Chem. -Int. Ed. 49, 2603-2606 (2010).
11. "Ultra-Sensitive Detection of TNF-α on Gold Nano-Patterned Protein Chip Formed via E-Beam Nanolithography by Total Internal Reflection Fluorescence Microscopy" Kyuseo Lee; Seungah Lee; Hyunung Yu; Seong Ho Kang, J. Nanosci. Nanotechnol. 10, 3228-3231 (2010).
10. "An Ultra-Sensitive Nanoarray Chip Based on Single-Molecule Sandwich Immunoassay and TIRFM for Protein Detection in Biologic Fluids" Seungah Lee; Nam-Pyo Cho; Jung Dong Kim; Hyungil Jung; Seong Ho Kang, Analyst 134, 933-938 (2009).
9. "Quantitative Analysis of Human Serum Leptin Using a Nanoarray Protein Chip Based on Single-Molecule Sandwich Immunoassay" Seungah Lee; Shinae Lee; Young-Ho Ko; Hyungil Jung; Jung Dong Kim; Joon Myong Song; Jaebum Choo; Seong Ho Kang, Talanta 78, 608-612 (2009).
8. "Real-Time Observations of Intracellular Mg2+ Signaling and Waves in Single Living Ventricular Myocyte Cell" Seungah Lee; Hee Gu Lee; Seong Ho Kang, Anal. Chem. 81(2), 538-542 (2009).
7. "Extra- and Intranuclear Dynamics and Distribution of Modified-PAMAM Polyplexes in Living Cells: A Single-Molecule Analysis" Seungah Lee; Kihoon Nam; Jong-Sang Park; Joon Sig Choi; Seong Kug Eo; Dong Jin Yoo; Seong Ho Kang, Bull. Korean Chem. Soc. 29, 1565-1568 (2008).
6. "Dual-Color Prism-Type TIRFM System for Direct Detection of Single-Biomolecules on Nanoarray Biochip" Seungah Lee; Bong Hyun Chung; Seong Ho Kang, Curr. Appl. Phys. 8, 700-705 (2008).
5. "Dexamethasone-Conjugated Low Molecular Weight Polyethylenimine as a Nucleus-Targeting Lipopolymer Gene Carrier" Yun Mi Bae; Hye Choi; Seungah Lee; Seong Ho Kang; Young Tae Kim; Kihoon Nam; Jong Sang Park; Minhyung Lee; Joon Sig Choi, Bioconjugate Chem. 18, 2029-2036 (2007).
4. "Combination of DIC with Prism-Type Total Internal Fluorescence Microscope for Direct Observation of PAMAM Dendrmer Nanoparticle as a Gene Delivery in Living Human Cells" Seungah Lee; Joon Sig Choi; Seong Ho Kang, J. Nanosci. Nanotechnol. 7, 3689-3694 (2007).
3. "Atypical Mobilities of Single Native DNA Molecules in Microchip Electrophoresis Revealed by Differential Interference Contrast Microscopy" Seong Ho Kang; Seungah Lee; Edward S. Yeung, Electrophoresis 27, 4149-4157 (2006).
2. "Differential Interference Contrast Microscopy for Real-Time Dynamics and Manipulation of Single Cells in Microchannels", Seungah Lee, Youngseong Kim, Seong Ho Kang, Microchem J. 80, 107-112 (2005).
1. "Direct Observation of Single Native DNA Molecules in a Microchannel by Differential Interference Contrast Microscopy", Seong Ho Kang, Seungah Lee and Edward S. Yeung, Anal. Chem. 76 (15), 4459-4464 (2004).
36. "Optical Super-Resolution Imaging of Surface Reactions" T. Chen, B. Dong, K. Chen, F. Zhao, S. Lee, P. Zhang, S. H. Kang, J. W. Ha, W. Xu, N. Fang, in revision by Chem. Rev. (2016).
35. "Ultrasensitive Detection of Alpha-fetoprotein by Total Internal Reflection Scattering-based Super-resolution Microscopy for Super-localization of Nano-immunoplasmonics" S. Ahn, P. Zhang, H. Yu, S. Lee, S. H. Kang, Anal. Chem., 88, 11070-11076 (2016).
34. "Direct Quantitative Screening of Influenza A Virus without DNA Amplification by Single-particle Dual-mode Total Internal Reflection Scattering" S. Lee, S. K. Chakkarapani, E. S. Yeung, S. H. Kang, Biosensors and Bioelectronics 87, 842–849 (2016).
33. "Augmented 3D Super-resolution of Fluorescence-free Nanoparticles Using Enhanced Dark-field Illumination Based on Wavelength-modulation and a Least- cubic Algorithm" P. Zhang, K. Kim, S. Lee, S. K. Chakkarapani, N. Fang, S. H. Kang, Scientific Reports 6, 2863; doi: 10.1038/srep32863 (2016).
32. "Quantitative Nanoimmunosensor based on Dark-field Illumination with Enhanced Sensitivity and On-off Switching using Scattering Signals" S. Lee, H. Nan, H. Yu, S. H. Kang, Biosensors and Bioelectronics 79, 709-714 (2016).
31. "Real-time Intracellular Mg2+ Signaling and Wave Propagation by Subdiffraction-Limit Super-Resolution Microscopy" S. K. Chakkarapani, S. Lee, G. Lee, S. H. Kang, Bull. Korean Chem. Soc. 36, 2589-2595 (2015).
30. "Super-resolution of Fluorescence-free Plasmonic Nanoparticles using Enhanced Dark-field Illumination based on Wavelength-modulation" P. Zhang, S. Lee, H. Yu, N. Fang, S. H. Kang, Scientific Reports 5, 11447; doi: 10.1038/srep11447 (2015).
29. "Plasmonic Metal Scattering Immunoassay by Total Internal Reflection Scattering Microscopy with Nanoscale Lateral Resolution” S. Lee, H. Yu, S. H. Kang, Chem. Commun., 51, 945-947 (2015).
28. "Ultra-sensitive Plasmonic Nanometal Scattering Immunosensor Based on Optical Control in Evanescent Field Layer" S. Lee, G. Park, Suresh Kumar Chakkarapani, Seong Ho Kang, Biosensors and Bioelectronics 63, 444-449 (2015).
27. "Fast-High-throughput Screening of H1N1 Virus by Parallel Detection with Multi-channel Microchip Electrophoresis" Peng Zhang; He Nan; Seungah Lee; Seong Ho Kang, submitted to "Microchip capillary electrophoresis" in the series Methods in Molecular Biology, Springer (2014).
26. Selective Fluorescence and Fluorescence-Free Detection of Single Biomolecules on Nanobiochips" Seungah Lee; Seong Ho Kang, J. Biomed. Nanotechnol 10, 2620–2640 (2014).
25. Fluorescent-free Detection on Nanobiochips based on Wavelength-Dependent Single Plasmonic Nanoparticles by Differential Interference Contrast Microscopy" Seungah Lee; Seong Ho Kang, Biosensors and Bioelectronics 60, 45-51 (2014).
24. "Selective Fluorescent-free Detection of Biomolecules on Nanobiochips by Wavelength Dependent-enhanced Dark Field Illumination" Seungah Lee; Hyunung Yu; Seong Ho Kang , Chem. Commun. 49, 8335-8337 (2013).
23. "Single C-Reactive Protein Molecule Detection on a Gold-Nanopatterned Chip Based on Total Internal Reflection Fluorescence" Yunmi Heo; Seungah Lee; Sang-Won Lee; Seong Ho Kang, accepted by BKCS (2013).
22. "Gold-nanopatterned Single Interleukin-6 Sandwich Immunoassay Chips with Zeptomolar Detection Capability Based on Evanescent Field-enhanced Fluorescence imaging" Seungah Lee; Seong Ho Kang, Analyst 138, 3478-3482 (2013).
21. "Single-molecule DNA Digestion in Various Alkanethiol-functionalized Gold Nanopores" Seungah Lee; Seong Ho Kang, Talanta 107, 297-303 (2013).
20. "Quenching Effect on Gold Nano-patterned Cardiac Troponin I Chip by Total Internal Reflection Fluorescence Microscopy" Seungah Lee; Seong Ho Kang, Talanta 104, 32-38 (2013).
19. "Single-molecule Sandwich Immunoassay for Quantification of Alpha-Fetoprotein Based on Evanescent Field-enhanced Fluorescence Imaging" Seungah Lee; Seong Ho Kang, J. Nanomaterials 2012, 1-7 (2012).
18. "Wide-range Quantification of Human Thyroid-stimulating Hormone Using Gold-nanopatterned Single-molecule Sandwich Immunoassay Chip" Seungah Lee; Seong Ho Kang, Talanta 99, 1030-1034 (2012).
17. "Photoswitchable Fluorescent Diarylethene in a Turn-on Mode for Live Cell Imaging" Shi-Chong Pang; Hyesook Hyun; Seungah Lee; Daeun Jang; Min Jae Lee; Seong Ho Kang; Kwang-Hyun Ahn, Chem. Commun. 48, 3745-3747 (2012).
16. "Integrated Optical Molecular Imaging System for Four-Dimensional Real-Time Detection in Living Single Cells" Seungah Lee; Seong Ho Kang, Biosens. Bioelectron. 31, 393-398 (2012).
15. "Enzyme Digestion of Entrapped Single-DNA Molecules in Nanopores" Seungah Lee; Seong Ho Kang; Edward S. Yeung, Talanta 85, 2135-2141 (2011).
14. "Native and Fluorescent Dye-Dependent Single-DNA Molecule Microchip Dynamics as Measured by Differential Interference Contrast Microscopy" Doori Oh; Seungah Lee; Seong Ho Kang, Chem. Commun. 47, 9137-9139 (2011).
13. "Digestion of Individual DNA Molecules by λ-Exonuclease at Liquid-Solid Interface" Seong Ho Kang; Seungah Lee; Edward S. Yeung, Analyst 135, 1759-1764 (2010).
12. "Detection of Single Enzyme Molecules Inside Nanopores Based on Chemiluminescence" Seong Ho Kang; Seungah Lee; Edward S. Yeung, Angew. Chem. 122, 2657-2660 (2010); Angew. Chem. -Int. Ed. 49, 2603-2606 (2010).
11. "Ultra-Sensitive Detection of TNF-α on Gold Nano-Patterned Protein Chip Formed via E-Beam Nanolithography by Total Internal Reflection Fluorescence Microscopy" Kyuseo Lee; Seungah Lee; Hyunung Yu; Seong Ho Kang, J. Nanosci. Nanotechnol. 10, 3228-3231 (2010).
10. "An Ultra-Sensitive Nanoarray Chip Based on Single-Molecule Sandwich Immunoassay and TIRFM for Protein Detection in Biologic Fluids" Seungah Lee; Nam-Pyo Cho; Jung Dong Kim; Hyungil Jung; Seong Ho Kang, Analyst 134, 933-938 (2009).
9. "Quantitative Analysis of Human Serum Leptin Using a Nanoarray Protein Chip Based on Single-Molecule Sandwich Immunoassay" Seungah Lee; Shinae Lee; Young-Ho Ko; Hyungil Jung; Jung Dong Kim; Joon Myong Song; Jaebum Choo; Seong Ho Kang, Talanta 78, 608-612 (2009).
8. "Real-Time Observations of Intracellular Mg2+ Signaling and Waves in Single Living Ventricular Myocyte Cell" Seungah Lee; Hee Gu Lee; Seong Ho Kang, Anal. Chem. 81(2), 538-542 (2009).
7. "Extra- and Intranuclear Dynamics and Distribution of Modified-PAMAM Polyplexes in Living Cells: A Single-Molecule Analysis" Seungah Lee; Kihoon Nam; Jong-Sang Park; Joon Sig Choi; Seong Kug Eo; Dong Jin Yoo; Seong Ho Kang, Bull. Korean Chem. Soc. 29, 1565-1568 (2008).
6. "Dual-Color Prism-Type TIRFM System for Direct Detection of Single-Biomolecules on Nanoarray Biochip" Seungah Lee; Bong Hyun Chung; Seong Ho Kang, Curr. Appl. Phys. 8, 700-705 (2008).
5. "Dexamethasone-Conjugated Low Molecular Weight Polyethylenimine as a Nucleus-Targeting Lipopolymer Gene Carrier" Yun Mi Bae; Hye Choi; Seungah Lee; Seong Ho Kang; Young Tae Kim; Kihoon Nam; Jong Sang Park; Minhyung Lee; Joon Sig Choi, Bioconjugate Chem. 18, 2029-2036 (2007).
4. "Combination of DIC with Prism-Type Total Internal Fluorescence Microscope for Direct Observation of PAMAM Dendrmer Nanoparticle as a Gene Delivery in Living Human Cells" Seungah Lee; Joon Sig Choi; Seong Ho Kang, J. Nanosci. Nanotechnol. 7, 3689-3694 (2007).
3. "Atypical Mobilities of Single Native DNA Molecules in Microchip Electrophoresis Revealed by Differential Interference Contrast Microscopy" Seong Ho Kang; Seungah Lee; Edward S. Yeung, Electrophoresis 27, 4149-4157 (2006).
2. "Differential Interference Contrast Microscopy for Real-Time Dynamics and Manipulation of Single Cells in Microchannels", Seungah Lee, Youngseong Kim, Seong Ho Kang, Microchem J. 80, 107-112 (2005).
1. "Direct Observation of Single Native DNA Molecules in a Microchannel by Differential Interference Contrast Microscopy", Seong Ho Kang, Seungah Lee and Edward S. Yeung, Anal. Chem. 76 (15), 4459-4464 (2004).
Presentations
40. "Immuno-nanoplasmonic Assay on Nanoscale: Diffraction-unlimited Imaging with TIRS Nanoscopy" S. Ahn, S. Lee, S. H. Kang. (oral presentation), The 118th General Meeting of the Korean Chemical Society, BEXCO, Busan, Korea, October 12-14, 2016
39. "Fluorescent-free Super-resolution Nanoscopy Based on Wavelength-dependent Plasmonic Scattering", S. H. Kang, P. Zhang, S. Lee. (oral presentation), 3rd International Multidisciplinary Microscopy and Microanalysis Congress & Exhibition (InterM2015), Sentido Lykia Resort, Oludeniz, Turkey, October 19-23, 2015
38. "Fluorescent-free Super-resolution Nanoscopy based on Enhanced Dark-field Illumination of Specific Localized Surface Plasmon Resonance Wavelengths" P. Zhang, S. Lee, S. H. Kang. (oral presentation), The 11th Analysis of Surface Symposium, Byunsan Daemyung Resort, Korea, October 6-8, 2015
37. "Immunoassay Chip based on Plasmonic Nanometal Scattering for Quantification Analysis of Human Thyroid-Stimulating Hormone" S. Lee, S. K. Chakkarapani, S. H. Kang. (poster), 2014 China‐Japan‐Korea Symposium on Analytical Chemistry, Northeastern University, Shenyang, China, August 22-25, 2014
36. "Three-dimensional Subdiffraction Limited Detection of Sandwich Immunoassay on Nanobiochip based on Wavelength-dependent Enhanced Dark Field Illumination" He Nan, Seungah Lee, Guenyoung Park, Seong Ho Kang. (poster), 2014 China‐Japan‐Korea Symposium on Analytical Chemistry, Northeastern University, Shenyang, China, August 22-25, 2014
35. "Single C-Reactive Protein Molecule Detection on Nanoarray Biochip Based on Total Internal Reflection Fluorescence" Yunmi Heo, Seungah Lee and Seong Ho Kang. (poster), The 111th General Meeting of the Korean Chemical Society of Goyang KINTEX, Korea, April 17-19, 2013
34. "Nanoarray Chip for High-Sensitive Immunodiagnosis of Human Thyrotropin" Seungah Lee and Seong Ho Kang. (poster), The 110th General Meeting of the Korean Chemical Society of Busan BEXCO, Korea, Oct 17-19, 2012
33. "Ultra-sensitive Gold Nano-patterned Protein Chip for Quantitative Analysis of Cardiac Troponin I" Seungah Lee and Seong Ho Kang. (poster), Smart Biochip Technology For Future Healthcare of Hanyang University ERIKA Campus, Korea, May 23-24, 2012
32. "Single-Molecule Nanoarray Protein Chip for Ultra-sensitive Immuno-diagnostic Assay" Seungah Lee and Seong Ho Kang. (poster), Smart Biochip Technology For Future Healthcare of Hanyang University ERIKA Campus, Korea, May 23-24, 2012
31. "Quenching Effect of Various TIRFM Systems on a Gold Nanoarray Biochip" Seungah Lee and Seong Ho Kang. (poster), The 48th National Meeting of the Korean Society of Analytical Sciences of Pyeongchang, Korea, May 17-18, 2012
30. "Enzymatic Digestion of Single-DNA Molecules in Nanopores" Seungah Lee and Seong Ho Kang. (Oral presentation), The 109th National Meeting of the Korean Chemical Society of IIsan KINTEX, Korea April 25-27, 2012
29. "Enzymatic Digestion of Single-DNA Molecules at Different Environment of Nanopores" Seungah Lee and Seong Ho Kang. (poster), The 109th National Meeting of the Korean Chemical Society of IIsan KINTEX, Korea April 25-27, 2012
28. "Real-Time Dynamics of Single Native-DNA Molecule in Microchip by Differential Interference Contrast Microscopy" Seungah Lee and Seong Ho Kang. (poster), The 108th National Meeting of the Korean Chemical Society of Daejeon, Korea September 28-30, 2011
27. "Geometrical Environment-Dependent Enzymatic Digestion of Single-DNA Molecule in Nanopores" Seungah Lee and Seong Ho Kang. (poster), The 108th National Meeting of the Korean Chemical Society of Daejeon, Korea September 28-30, 2011
26. "Direct Observation of Real-Time Enzymatic Digestion of Single-DNA Molecule" Seungah Lee and Seong Ho Kang. (poster), The 107th National Meeting of the Korean Chemical Society of Jeju, Korea April 28-29, 2011
25. "Ultra-Sensitive Nanoarray Protein Chip Based on TIRFM for Detection of Human Leptin Protein" Seungah Lee, Kyuseo Lee and Seong Ho Kang. (poster), 15th Anniversary Meeting for Korean Society of Photoscience, Jeju, Korea, September 25-28, 2008
24. "Ultra-Sensitive Detection of Tumor Necrosis Factor-alpha on Gold Nano-patterned Protein Chip by Total Internal Reflection Fluorescence Microscopy" Kyuseo Lee, Seungah Lee and Seong Ho Kang. (poster), 15th Anniversary Meeting for Korean Society of Photoscience, Jeju, Korea, September 25-28, 2008
23. "Highly Sensitive Nanoarray Protein Chip for the Diagnosis of Human Cytokine Cancer Based on TIRF Immuno-Assay" Seungah Lee and Seong Ho Kang. (poster), The 10th Regional Meeting of the Korean Chemical Society of Gwangju, Chonnam, and Jeonbuk, Jeonju University Hope Hall, June 13, 2008
22. "Zeptomolar Detection of Nanoarray Protein Chip Based on Total Internal Reflection Fluorescence Microscopy" Seungah Lee and Seong Ho Kang. (Oral presentation), The 3rd ISBBN, China, June 8-11, 2008
21. "Nanoarray Biochip Application Using Single-Molecule Detection Technique" Seong Ho Kang, Seungah Lee, Kyuseo Lee. (poster), Symposium on New Frontiers of Bio-Imaging and Micro-Separation, Ames, Iowa, USA, May 24-25, 2008
20. "Ultra-Sensitive Nanoarray Protein Chip for the Diagnosis of Human Cytokine Cancer" Seungah Lee and Seong Ho Kang. (poster), The 101st National Meeting of the Korean Chemical Society of IIsan KINTEX, April 17-18, 2008
19. "Real-Time Visualizing of Intracellular Mg2+ Ion Signaling in Living Cell on Single-Molecule Level Using Total Internal Reflection Fluorescence Microscope" Seungah Lee, Yong-Geun Kwak and Seong Ho Kang. (poster), The 9th Asian Conference on Analytical Science, Novermber 4-8, 2007
18. "Real-Time Wave Phenomenon of Intracellular Magnesium Ion in Living Rat Ventricular Myocytes on Single-Molecule Level" Seungah Lee and Seong Ho Kang. (poster), 대한화학회 전기화학-분석화학 분과 합동 심포지엄, 대천 충남대학교 연수원, June 28-29, 2007
17. "Microchip-Based Capillary Gel Electrophoresis with Programmed Field Strength Gradients for Ultra-fast Detection of Salmonella by rfbS Allele-Specific PCR". Seungah Lee, Won Lee and Seong Ho Kang. (poster), Dalian International Symposia and Exhibition on Chromatography Dalian, China, June 4-7, 2007
16. "Real-Time Visualizing of Mg2+ Spark in Living Cells Using Total Internal Reflection Fluorescence Microscope" Seungah Lee, Seong Ho Kang. (poster), The 9th Annual Meeting of the Korean Chemical Society of Gwangju.Chonnam.Chonbuk, Chosun University, June 1, 2007
15. "Real-Time Visualizing the Dynamics and Localizations of Two Modified-PAMAM Nanoparticles as a Gene-Deliver in Living Cells" Seungah Lee and Seong Ho Kang. (poster), Symposium of the Korean Chemical Society of Seoul COEX, April 19-20, 2007
14. "Comparison of Real-Time Localization of DNA-Nanoparticle Complexes in Living Cells Using Combined DIC-TIRFM System and 3D Confocal Microscope" Seungah Lee, Seong Ho Kang (poster), 2006 International Conference on Nano Science and Nano Technology, Gwangju Nanotechnology Integration Center Korea Institute of Industrial Technology, Gwangju, Korea December 07-08, 2006
13. "Dual-Color Prism-Type TIRFM System for Direct Detection of Single-Biomolecules on Nanoarray Biochips" Seungah Lee, Bong Hyun Chung, Seong Ho Kang (poster), 2006 International Nanotech Symposium of KINTEX, August 30, 2006
12. "Polyethylenimine Dendrimer-Grafted-Examethasone as a Nucleus Targeting Water-Soluble Lipopolymer Gene Carrier" Yun Mi Bae; Hye Choi; Seungah Lee, Seong Ho Kang, Minhyung Lee, Joon Sig Choi (poster), 33rd Annual Meeting and Exposition of the Controlled Release Society, Austria Center/Vienna, Austria, July 22-26, 2006
11. "Expression of Modified Dendrimer Imaging in Living Cells Using a Combined System of DIC and Two-Color TIRFM" Seungah Lee; Joon Sig Choi; Seong Ho Kang (poster), The Annual Meeting of the Korean Chemical Society of Chonbuk·Chonnam·Gwangju, June 2, 2006
10. "Novel Combinational System of DIC and TIRF Microscope of the Direct Detection of Single Nanoparicles in Living Cells" Seungah Lee; Seong Ho Kang (poster), The Annual Meeting of the Korean Chemical Society of Chonbuk·Chonnam·Gwangju, June 2, 2006
9. "Direct Observation of PAMAM-DNA Nanoparticles in Living Cells Using a Combinational System of DIC and TIRFM" Seungah Lee, Seong Ho Kang. (Oral presentation), The Korean Society of analytical Sciences, Mokpo University, May 18, 2006
8. "Internalization of Nanoparticle-DNA Complexes in Living Human Embryonic Kidney 293 Cell Using a Combinational System of DIC and TIRF Microscope" Seungah Lee, Seong Ho Kang. (poster), 8th Asian Conference on Analytical Science, Taiwan, October 16, 2005
7. "Real-Time Dynamics of Different Size Single Native DNA Molecules in a Microchip by Transmitted DIC Microscopy" Seungah Lee, Seong Ho Kang. (poster), 2005 International Nanotech Symposium of KINTEX, August 24-26, 2005
6. "Transmitted-DIC for Direct Detection and Manipulation of Different Size Single-Native DNA Molecules in Microchip" Seungah Lee, Sang-Kyu Park and Seong Ho Kang. (poster), Symposium of the Korean Chemical Society of Ilsung Solak Condo, Gangwon, June 24-25, 2005
5. "Real-Time Monitoring of Different Size Single-Native DNA Molecules in Microchip by Nomarski-DIC" Seungah Lee and Seong Ho Kang. (poster), The 95th National Meeting of the Korean Chemical Society of Inha University, April 22-23, 2005
4. "Real-Time Dynamics of Single-Native DNA Molecules in Microfluidic Chip Using Nomarski-DIC" Seungah Lee and Seong Ho Kang. (poster), The 5th Asia-Pacific International Symposium on Microscale Separations and Analysis, Seoul, Korea, December 5-8, 2004
3. "Single-Native DNA Molecules Detection in Microfluidic Chip by Nomarski-Differential Interference Contrast" Seungah Lee and Seong Ho Kang. (poster), The 6th annual meeting of the Korean Chemical Society of Chonbuk·Chonnam·Gwangju, June 4, 2004
2. "Nomarski-DIC for the Single-Cell Detection and Manipulation at Nanofluidics" Seungah Lee and Seong Ho Kang. (poster), The 92nd National Meeting of the Korean Chemical Society of Busan, October 16-17, 2003
1. "Real-Time Monitoring of Single-Cell Molecules at Nanofluidics by Nomarski-Differential Interference Contrast" Seungah Lee and Seong Ho Kang. (poster), Symposium of the Korean Chemical Society of Byunsan (대한화학회 전기화학-분석화학 분과 합동 심포지엄), June 20, 2003
39. "Fluorescent-free Super-resolution Nanoscopy Based on Wavelength-dependent Plasmonic Scattering", S. H. Kang, P. Zhang, S. Lee. (oral presentation), 3rd International Multidisciplinary Microscopy and Microanalysis Congress & Exhibition (InterM2015), Sentido Lykia Resort, Oludeniz, Turkey, October 19-23, 2015
38. "Fluorescent-free Super-resolution Nanoscopy based on Enhanced Dark-field Illumination of Specific Localized Surface Plasmon Resonance Wavelengths" P. Zhang, S. Lee, S. H. Kang. (oral presentation), The 11th Analysis of Surface Symposium, Byunsan Daemyung Resort, Korea, October 6-8, 2015
37. "Immunoassay Chip based on Plasmonic Nanometal Scattering for Quantification Analysis of Human Thyroid-Stimulating Hormone" S. Lee, S. K. Chakkarapani, S. H. Kang. (poster), 2014 China‐Japan‐Korea Symposium on Analytical Chemistry, Northeastern University, Shenyang, China, August 22-25, 2014
36. "Three-dimensional Subdiffraction Limited Detection of Sandwich Immunoassay on Nanobiochip based on Wavelength-dependent Enhanced Dark Field Illumination" He Nan, Seungah Lee, Guenyoung Park, Seong Ho Kang. (poster), 2014 China‐Japan‐Korea Symposium on Analytical Chemistry, Northeastern University, Shenyang, China, August 22-25, 2014
35. "Single C-Reactive Protein Molecule Detection on Nanoarray Biochip Based on Total Internal Reflection Fluorescence" Yunmi Heo, Seungah Lee and Seong Ho Kang. (poster), The 111th General Meeting of the Korean Chemical Society of Goyang KINTEX, Korea, April 17-19, 2013
34. "Nanoarray Chip for High-Sensitive Immunodiagnosis of Human Thyrotropin" Seungah Lee and Seong Ho Kang. (poster), The 110th General Meeting of the Korean Chemical Society of Busan BEXCO, Korea, Oct 17-19, 2012
33. "Ultra-sensitive Gold Nano-patterned Protein Chip for Quantitative Analysis of Cardiac Troponin I" Seungah Lee and Seong Ho Kang. (poster), Smart Biochip Technology For Future Healthcare of Hanyang University ERIKA Campus, Korea, May 23-24, 2012
32. "Single-Molecule Nanoarray Protein Chip for Ultra-sensitive Immuno-diagnostic Assay" Seungah Lee and Seong Ho Kang. (poster), Smart Biochip Technology For Future Healthcare of Hanyang University ERIKA Campus, Korea, May 23-24, 2012
31. "Quenching Effect of Various TIRFM Systems on a Gold Nanoarray Biochip" Seungah Lee and Seong Ho Kang. (poster), The 48th National Meeting of the Korean Society of Analytical Sciences of Pyeongchang, Korea, May 17-18, 2012
30. "Enzymatic Digestion of Single-DNA Molecules in Nanopores" Seungah Lee and Seong Ho Kang. (Oral presentation), The 109th National Meeting of the Korean Chemical Society of IIsan KINTEX, Korea April 25-27, 2012
29. "Enzymatic Digestion of Single-DNA Molecules at Different Environment of Nanopores" Seungah Lee and Seong Ho Kang. (poster), The 109th National Meeting of the Korean Chemical Society of IIsan KINTEX, Korea April 25-27, 2012
28. "Real-Time Dynamics of Single Native-DNA Molecule in Microchip by Differential Interference Contrast Microscopy" Seungah Lee and Seong Ho Kang. (poster), The 108th National Meeting of the Korean Chemical Society of Daejeon, Korea September 28-30, 2011
27. "Geometrical Environment-Dependent Enzymatic Digestion of Single-DNA Molecule in Nanopores" Seungah Lee and Seong Ho Kang. (poster), The 108th National Meeting of the Korean Chemical Society of Daejeon, Korea September 28-30, 2011
26. "Direct Observation of Real-Time Enzymatic Digestion of Single-DNA Molecule" Seungah Lee and Seong Ho Kang. (poster), The 107th National Meeting of the Korean Chemical Society of Jeju, Korea April 28-29, 2011
25. "Ultra-Sensitive Nanoarray Protein Chip Based on TIRFM for Detection of Human Leptin Protein" Seungah Lee, Kyuseo Lee and Seong Ho Kang. (poster), 15th Anniversary Meeting for Korean Society of Photoscience, Jeju, Korea, September 25-28, 2008
24. "Ultra-Sensitive Detection of Tumor Necrosis Factor-alpha on Gold Nano-patterned Protein Chip by Total Internal Reflection Fluorescence Microscopy" Kyuseo Lee, Seungah Lee and Seong Ho Kang. (poster), 15th Anniversary Meeting for Korean Society of Photoscience, Jeju, Korea, September 25-28, 2008
23. "Highly Sensitive Nanoarray Protein Chip for the Diagnosis of Human Cytokine Cancer Based on TIRF Immuno-Assay" Seungah Lee and Seong Ho Kang. (poster), The 10th Regional Meeting of the Korean Chemical Society of Gwangju, Chonnam, and Jeonbuk, Jeonju University Hope Hall, June 13, 2008
22. "Zeptomolar Detection of Nanoarray Protein Chip Based on Total Internal Reflection Fluorescence Microscopy" Seungah Lee and Seong Ho Kang. (Oral presentation), The 3rd ISBBN, China, June 8-11, 2008
21. "Nanoarray Biochip Application Using Single-Molecule Detection Technique" Seong Ho Kang, Seungah Lee, Kyuseo Lee. (poster), Symposium on New Frontiers of Bio-Imaging and Micro-Separation, Ames, Iowa, USA, May 24-25, 2008
20. "Ultra-Sensitive Nanoarray Protein Chip for the Diagnosis of Human Cytokine Cancer" Seungah Lee and Seong Ho Kang. (poster), The 101st National Meeting of the Korean Chemical Society of IIsan KINTEX, April 17-18, 2008
19. "Real-Time Visualizing of Intracellular Mg2+ Ion Signaling in Living Cell on Single-Molecule Level Using Total Internal Reflection Fluorescence Microscope" Seungah Lee, Yong-Geun Kwak and Seong Ho Kang. (poster), The 9th Asian Conference on Analytical Science, Novermber 4-8, 2007
18. "Real-Time Wave Phenomenon of Intracellular Magnesium Ion in Living Rat Ventricular Myocytes on Single-Molecule Level" Seungah Lee and Seong Ho Kang. (poster), 대한화학회 전기화학-분석화학 분과 합동 심포지엄, 대천 충남대학교 연수원, June 28-29, 2007
17. "Microchip-Based Capillary Gel Electrophoresis with Programmed Field Strength Gradients for Ultra-fast Detection of Salmonella by rfbS Allele-Specific PCR". Seungah Lee, Won Lee and Seong Ho Kang. (poster), Dalian International Symposia and Exhibition on Chromatography Dalian, China, June 4-7, 2007
16. "Real-Time Visualizing of Mg2+ Spark in Living Cells Using Total Internal Reflection Fluorescence Microscope" Seungah Lee, Seong Ho Kang. (poster), The 9th Annual Meeting of the Korean Chemical Society of Gwangju.Chonnam.Chonbuk, Chosun University, June 1, 2007
15. "Real-Time Visualizing the Dynamics and Localizations of Two Modified-PAMAM Nanoparticles as a Gene-Deliver in Living Cells" Seungah Lee and Seong Ho Kang. (poster), Symposium of the Korean Chemical Society of Seoul COEX, April 19-20, 2007
14. "Comparison of Real-Time Localization of DNA-Nanoparticle Complexes in Living Cells Using Combined DIC-TIRFM System and 3D Confocal Microscope" Seungah Lee, Seong Ho Kang (poster), 2006 International Conference on Nano Science and Nano Technology, Gwangju Nanotechnology Integration Center Korea Institute of Industrial Technology, Gwangju, Korea December 07-08, 2006
13. "Dual-Color Prism-Type TIRFM System for Direct Detection of Single-Biomolecules on Nanoarray Biochips" Seungah Lee, Bong Hyun Chung, Seong Ho Kang (poster), 2006 International Nanotech Symposium of KINTEX, August 30, 2006
12. "Polyethylenimine Dendrimer-Grafted-Examethasone as a Nucleus Targeting Water-Soluble Lipopolymer Gene Carrier" Yun Mi Bae; Hye Choi; Seungah Lee, Seong Ho Kang, Minhyung Lee, Joon Sig Choi (poster), 33rd Annual Meeting and Exposition of the Controlled Release Society, Austria Center/Vienna, Austria, July 22-26, 2006
11. "Expression of Modified Dendrimer Imaging in Living Cells Using a Combined System of DIC and Two-Color TIRFM" Seungah Lee; Joon Sig Choi; Seong Ho Kang (poster), The Annual Meeting of the Korean Chemical Society of Chonbuk·Chonnam·Gwangju, June 2, 2006
10. "Novel Combinational System of DIC and TIRF Microscope of the Direct Detection of Single Nanoparicles in Living Cells" Seungah Lee; Seong Ho Kang (poster), The Annual Meeting of the Korean Chemical Society of Chonbuk·Chonnam·Gwangju, June 2, 2006
9. "Direct Observation of PAMAM-DNA Nanoparticles in Living Cells Using a Combinational System of DIC and TIRFM" Seungah Lee, Seong Ho Kang. (Oral presentation), The Korean Society of analytical Sciences, Mokpo University, May 18, 2006
8. "Internalization of Nanoparticle-DNA Complexes in Living Human Embryonic Kidney 293 Cell Using a Combinational System of DIC and TIRF Microscope" Seungah Lee, Seong Ho Kang. (poster), 8th Asian Conference on Analytical Science, Taiwan, October 16, 2005
7. "Real-Time Dynamics of Different Size Single Native DNA Molecules in a Microchip by Transmitted DIC Microscopy" Seungah Lee, Seong Ho Kang. (poster), 2005 International Nanotech Symposium of KINTEX, August 24-26, 2005
6. "Transmitted-DIC for Direct Detection and Manipulation of Different Size Single-Native DNA Molecules in Microchip" Seungah Lee, Sang-Kyu Park and Seong Ho Kang. (poster), Symposium of the Korean Chemical Society of Ilsung Solak Condo, Gangwon, June 24-25, 2005
5. "Real-Time Monitoring of Different Size Single-Native DNA Molecules in Microchip by Nomarski-DIC" Seungah Lee and Seong Ho Kang. (poster), The 95th National Meeting of the Korean Chemical Society of Inha University, April 22-23, 2005
4. "Real-Time Dynamics of Single-Native DNA Molecules in Microfluidic Chip Using Nomarski-DIC" Seungah Lee and Seong Ho Kang. (poster), The 5th Asia-Pacific International Symposium on Microscale Separations and Analysis, Seoul, Korea, December 5-8, 2004
3. "Single-Native DNA Molecules Detection in Microfluidic Chip by Nomarski-Differential Interference Contrast" Seungah Lee and Seong Ho Kang. (poster), The 6th annual meeting of the Korean Chemical Society of Chonbuk·Chonnam·Gwangju, June 4, 2004
2. "Nomarski-DIC for the Single-Cell Detection and Manipulation at Nanofluidics" Seungah Lee and Seong Ho Kang. (poster), The 92nd National Meeting of the Korean Chemical Society of Busan, October 16-17, 2003
1. "Real-Time Monitoring of Single-Cell Molecules at Nanofluidics by Nomarski-Differential Interference Contrast" Seungah Lee and Seong Ho Kang. (poster), Symposium of the Korean Chemical Society of Byunsan (대한화학회 전기화학-분석화학 분과 합동 심포지엄), June 20, 2003
Patents
• "파장-의존성 미분간섭대비 현미경법을 이용한 표적 생체분자의 비형광 검출방법 및 시스템 (A method and system for fluorecence-free detection of a target biomolecule using wavelength-dependent differential interference contrast microscopy)" Seong Ho Kang; Seungah Lee, 출원KR 1020130144892
• "파장-의존성 플라즈몬 공명산란을 이용한 나노바이오칩 키트 및 이를 이용한 생체분자의 검출 방법 (Nanopatterned biochip kit based on wavelength-dependent plasmon resonance scattering and biomolecule detection method using the same)" Seong Ho Kang; Seungah Lee, 공개번호 KR 1020130045762, 등록일자 2014. 12. 02
• "다중-표적분자 동시검출용 고감도 복합-나노바이오칩 및 이를 이용한 질병진단의 정보제공방법 (High sensitive combined-nanobiochip for simultaneous detection of multi-biomolecule markers and using the same information providing method of disease diagnosis)" Seong Ho Kang; Seungah Lee, 공개번호 1020110044261, 공개일자 2014. 08. 13
• "살아 있는 세포 내 단일분자 또는 나노입자의 실시간 검출을 위한 다기능 복합 이미지 검출 장치" Seong Ho Kang; Seungah Lee, 공개번호 1020120124904, 공개일자 2012. 11. 14.
• “Method for Single-Molecule Detection in Cell Using a Combination of DIC Microscopy and TIRFM and the Apparatus therefor for Measuring One or More Gene or a Single Biomolecule such as Protein in Cell" 미분간섭효과 현미경 기술과 전반사형광 현미경 기술을 결합하여 이용한 세포 내 단일 분자의 검출 방법 및 그 장치" Seong Ho Kang; Seungah Lee, 출원 2005-0077860호, KR 20070023354, (등록일: 2007. 12. 05. 제 10-0785040호)
• "Method for Detecting Biomolecules Using Differential Interference Contrast Microscopy without Dyeing DNA with Fluorescent Dyes and Microchip for the Same Method" 미분간섭현미경을 이용한 생체분자검출방법 및 검출시 사용되는 마이크로칩" Seong Ho Kang; Seungah Lee; Jun Keun Chang; Chan Il Chung, 출원 2004-0074647, KR 20060025813, (등록일: 2006. 12. 05. 제 10-0656564호)
• "파장-의존성 플라즈몬 공명산란을 이용한 나노바이오칩 키트 및 이를 이용한 생체분자의 검출 방법 (Nanopatterned biochip kit based on wavelength-dependent plasmon resonance scattering and biomolecule detection method using the same)" Seong Ho Kang; Seungah Lee, 공개번호 KR 1020130045762, 등록일자 2014. 12. 02
• "다중-표적분자 동시검출용 고감도 복합-나노바이오칩 및 이를 이용한 질병진단의 정보제공방법 (High sensitive combined-nanobiochip for simultaneous detection of multi-biomolecule markers and using the same information providing method of disease diagnosis)" Seong Ho Kang; Seungah Lee, 공개번호 1020110044261, 공개일자 2014. 08. 13
• "살아 있는 세포 내 단일분자 또는 나노입자의 실시간 검출을 위한 다기능 복합 이미지 검출 장치" Seong Ho Kang; Seungah Lee, 공개번호 1020120124904, 공개일자 2012. 11. 14.
• “Method for Single-Molecule Detection in Cell Using a Combination of DIC Microscopy and TIRFM and the Apparatus therefor for Measuring One or More Gene or a Single Biomolecule such as Protein in Cell" 미분간섭효과 현미경 기술과 전반사형광 현미경 기술을 결합하여 이용한 세포 내 단일 분자의 검출 방법 및 그 장치" Seong Ho Kang; Seungah Lee, 출원 2005-0077860호, KR 20070023354, (등록일: 2007. 12. 05. 제 10-0785040호)
• "Method for Detecting Biomolecules Using Differential Interference Contrast Microscopy without Dyeing DNA with Fluorescent Dyes and Microchip for the Same Method" 미분간섭현미경을 이용한 생체분자검출방법 및 검출시 사용되는 마이크로칩" Seong Ho Kang; Seungah Lee; Jun Keun Chang; Chan Il Chung, 출원 2004-0074647, KR 20060025813, (등록일: 2006. 12. 05. 제 10-0656564호)
Research of interest
Single Molecule Detection & Nanobio Chip
